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plasmid constructs  (Addgene inc)


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    Structured Review

    Addgene inc plasmid constructs
    Plasmid Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mscv/MSCV-beta-catenin-IRES-GFP+(Plasmid+%2314717)/pm41931626-315-9-16
    Average 93 stars, based on 13 article reviews
    plasmid constructs - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Over Expression:

    Article Title: Epigenetic regulation of Fanconi anemia genes implicates PRMT5 blockage as a strategy for tumor chemosensitization
    Article Snippet: .. Briefly, overexpression of PRMT5 was obtained using lentiviral vector pLX304 (Addgene #25890), and restoration of MTAP in U118MG cell line was obtained using MSCV (Addgene #24828). ..

    Article Title: The Host miR-17-92 Cluster Negatively Regulates Mouse Mammary Tumor Virus (MMTV) Replication Primarily Via Cluster Member miR-92a.
    Article Snippet: .. We would like to thank Prof. Jeffery M. Rosen, Baylor College of Medicine, Houston, TX USA for the gift of HC11 cells, and the Lin He Lab (Lin He, Professor of Cell Biology, Development & Physiology University of California, Berkeley CA, USA) for sharing the miRNA overexpression plasmids: MSCV, MSCV-mir-17-92, MSCV-19a20-19b, MSCV-mir-92, MLS-mir-17-92, MLS- mir17-19b, MLS-mir-17-92-Mut92 (Addgene plasmid #: 24828, 64100, 24827, 64092, 64090, 64089, & 64094). ..

    Transfection:

    Article Title: Olaparib-induced Apoptosis Through EBNA1-ATR-p38 MAPK Signaling Pathway in Epstein-Barr Virus-positive Gastric Cancer Cells.
    Article Snippet: Background: Epstein-Barr virus (EBV)-associated gastric cancer has been identified as a cancer subtype with definitive clinical and molecular characteristics.. Although olaparib, a poly ADP ribose polymerase (PARP) inhibitor, is considered a potential effective agent for gastric cancer, the effect and underlying mechanism of olaparib on gastric cancer depending on EBV infection is not fully understood.. Materials and Methods: EBV-positive SNU719 and EBV-negative SNU638 gastric cancer cell lines were used to identify the effects of olaparib using the trypan blue exclusion method and annexin V staining assay.

    Article Title: Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
    Article Snippet: .. SiHa and CaSki cells were seeded into 6-well plates at a density of 0.5 × 10 6 cells per well and on the next day transfected with 30 μM MSCV (Addgene, Plasmid #41033, depositing lab: Wade Harpe) or MSCVBARF1 (Addgene, Plasmid #37922, depositing lab: Karl Munger) plasmids using Lipofectamine ® 2000 (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s protocol. .. Cells were maintained in culture medium without antibiotics for 12–18 h, after which transfected cells were selected by addition of 0.2 μg/mL puromycin for seven days (Gibco, Carlsbad, CA, USA).

    Article Title: Epstein-Barr Virus BARF1 Is Expressed in Lung Cancer and Is Associated with Cancer Progression
    Article Snippet: .. Cells were seeded into 6-well plates at a density of 1.5 × 10 5 cells per well and, on the following day, were transfected with 1.5 μg MSCV (Addgene, Plasmid #41033, depositing name: Wade Harpe) or MSCVBARF1 (Addgene, Plasmid #37922, depositing lab: Karl Munger) plasmids using FuGENE ® 6 transfection reagent (Promega, Madison, WI, USA) following the manufacturer’s protocol. .. Transfected A549 and BEAS-2B cells were maintained in culture medium without antibiotics for 12–18 h and selected with 0.3 μg/mL puromycin for two weeks (Gibco, Carlsbad, CA, USA), as previously described in Muñoz JP et al. (2012) [ ] and Peña N et al. (2015) [ ].

    Plasmid Preparation:

    Article Title: Olaparib-induced Apoptosis Through EBNA1-ATR-p38 MAPK Signaling Pathway in Epstein-Barr Virus-positive Gastric Cancer Cells.
    Article Snippet: Background: Epstein-Barr virus (EBV)-associated gastric cancer has been identified as a cancer subtype with definitive clinical and molecular characteristics.. Although olaparib, a poly ADP ribose polymerase (PARP) inhibitor, is considered a potential effective agent for gastric cancer, the effect and underlying mechanism of olaparib on gastric cancer depending on EBV infection is not fully understood.. Materials and Methods: EBV-positive SNU719 and EBV-negative SNU638 gastric cancer cell lines were used to identify the effects of olaparib using the trypan blue exclusion method and annexin V staining assay.

    Article Title: Characterization of High-Risk HPV/EBV Co-Presence in Pre-Malignant Cervical Lesions and Squamous Cell Carcinomas
    Article Snippet: .. SiHa and CaSki cells were seeded into 6-well plates at a density of 0.5 × 10 6 cells per well and on the next day transfected with 30 μM MSCV (Addgene, Plasmid #41033, depositing lab: Wade Harpe) or MSCVBARF1 (Addgene, Plasmid #37922, depositing lab: Karl Munger) plasmids using Lipofectamine ® 2000 (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s protocol. .. Cells were maintained in culture medium without antibiotics for 12–18 h, after which transfected cells were selected by addition of 0.2 μg/mL puromycin for seven days (Gibco, Carlsbad, CA, USA).

    Article Title: The Host miR-17-92 Cluster Negatively Regulates Mouse Mammary Tumor Virus (MMTV) Replication Primarily Via Cluster Member miR-92a.
    Article Snippet: .. We would like to thank Prof. Jeffery M. Rosen, Baylor College of Medicine, Houston, TX USA for the gift of HC11 cells, and the Lin He Lab (Lin He, Professor of Cell Biology, Development & Physiology University of California, Berkeley CA, USA) for sharing the miRNA overexpression plasmids: MSCV, MSCV-mir-17-92, MSCV-19a20-19b, MSCV-mir-92, MLS-mir-17-92, MLS- mir17-19b, MLS-mir-17-92-Mut92 (Addgene plasmid #: 24828, 64100, 24827, 64092, 64090, 64089, & 64094). ..

    Article Title: Epstein-Barr Virus BARF1 Is Expressed in Lung Cancer and Is Associated with Cancer Progression
    Article Snippet: .. Cells were seeded into 6-well plates at a density of 1.5 × 10 5 cells per well and, on the following day, were transfected with 1.5 μg MSCV (Addgene, Plasmid #41033, depositing name: Wade Harpe) or MSCVBARF1 (Addgene, Plasmid #37922, depositing lab: Karl Munger) plasmids using FuGENE ® 6 transfection reagent (Promega, Madison, WI, USA) following the manufacturer’s protocol. .. Transfected A549 and BEAS-2B cells were maintained in culture medium without antibiotics for 12–18 h and selected with 0.3 μg/mL puromycin for two weeks (Gibco, Carlsbad, CA, USA), as previously described in Muñoz JP et al. (2012) [ ] and Peña N et al. (2015) [ ].



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